Sensitive thyrotrophin assays

Sensitive thyrotrophin assays: excellent when properly used.

The Role of Thyrotrophin Receptor Antibody Assays in Graves' Disease

Thyrotrophin receptor antibodies (TRAb) exist as stimulating or blocking antibodies in the serum (neutral TRAb have been identified recently). The clinical features of GD occur when stimulating TRAb predominate. But the relationship of TRAb to clinical phenotype and outcome is not clear when current assay methods are used. Therefore no consensus exists about its utility in diagnosing and predic...

Proximity assays for sensitive quantification of proteins

Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein-protein interact...

How sensitive are immunometric assays for thyrotropin?

The usual method for calculation of the "sensitivity" of thyrotropin immunometric assays is multireplicate analysis of the zero analyte standard. Although this is a statistically valid estimate of the scatter likely to be found in the response variable, it is unrelated to normal analytical practice (usually analysis in duplicate) and estimates intra-assay errors only. This study was designed to...

An Accelerated Approach to Sensitive ADA Assays

The performance of an antibody in an assay often correlates with its affinity to the antigen. The ability to specifically select for antibodies on the basis of binding strength is therefore an important element of antibody assay development. Affinity or koff-rate determination can be carried out on several platforms; for example a rough ranking by ELISA, or quantitative screening using a techno...